FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598829
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT49930.312291058018087No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT49890.31204087491532867No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGAAGATACA46370.2900247618725955No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC45100.28208144836001847No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC40650.25424857817815416No Hit
GTGTATTACTGTGCGAGAGCCGTTTGGTTCGGGGAGTTATGGGACTACTG38500.24080123640489384No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32930.2059632393457962No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG31810.19895811246856293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31430.1965813729923588No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29700.18576095379806096No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28850.1804445628644464No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA28420.17775509450979438No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT27380.17125033383807775No Hit
CTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAAAAGCCAC27220.17024960142704443No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGTGAGAAATACTA27050.1691863232403215No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC26950.1685608654834257No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC26760.16737249574532362No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTGGCTTTTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAAGGG26320.16462048161498197No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG26060.1629942914470528No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG25980.16249392524153616No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT25510.15955427378412576No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25150.15730262585930077No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24500.15323715043947791No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT24080.15061022786051542No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT24060.15048513630913626No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC24010.15017240743068835No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT23870.14929676657103416No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA23720.14835857993569043No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23350.14604438623517588No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT23130.14466837917000505No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT22850.14291709745069672No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGAAGATA22170.1386639847038051No Hit
GTATAACAGTGCCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20850.1304079423127802No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA20760.1298450303315739No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG20570.12865666059347186No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA20560.12859411481778227No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA20460.12796865706088645No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG20420.1277184739581281No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTG20340.12721810775261144No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG20210.12640501266864687No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT20110.12577955491175102No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20110.12577955491175102No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA19870.12427845629520105No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19720.12334026965985731No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19390.12127625906210107No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18940.11846169915606984No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC18760.11733587519365736No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG18690.11689805476383028No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC18680.1168355089881407No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA18670.1167729632124511No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC18380.11495913571745321No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17870.11176930115728448No Hit
GCATAGATCAGGAGCCTAGGACCTTTACCTGGTTTCTGCTGATACCAGGC17860.11170675538159491No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC17820.11145657227883657No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA17690.11064347719487198No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTT17610.11014311098935534No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17210.10764127996177203No Hit
GTATTACTGTGCGAGAGCCGTTTGGTTCGGGGAGTTATGGGACTACTGGGGCCAGGGAATCCTGGTCACCGTCGC17000.1063278186722908No Hit
GTTATGGGACTACTGGGGCCAGGGAATCCTGGTCACCGTCGCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCC16940.1059525440181533No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACGTCTTCAGGCTGCAGGCTGGTGATGGTGAGAATGAAATCTGTC16800.10507690315849913No Hit
CGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGT16670.10426380807453456No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG16430.10276270945798456No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16410.10263761790660539No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16400.10257507213091582No Hit
GTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGA16330.10213725170108874No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC16180.101199065065745No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC16060.10044851575747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4050.046.5778271
GTATCAA21800.035.3995361
ATATAGC553.376535E-431.1723966
TATCAAC26400.029.3549232
ATCAACG28050.027.7469433
CAACGCA28200.027.7235345
AACGCAG28350.027.6978026
TCAACGC28400.027.4058484
AGAGTAC29300.026.21460211
TCTGGAG19550.025.93320870
ACCGTCG20950.025.75149770
CAGAGTA30050.025.67443510
CGCAGAG30350.025.6466128
GCAGAGT31350.024.7191649
TGTTAGA8550.023.56698270
AGCGCTG11050.023.2735085
GTGCTTT10700.023.07339913
TTAACGG902.1422927E-422.97544935
TGCACAG15050.022.89010470
GGGAACG13700.022.77346270
Produced by FastQC (version 0.11.5)