FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021838
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17430.1705749835101063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTTAG200.007412297751.6552731
TAATACA200.00741947451.642632
TGTATAA1050.042.6256642
GTGTATA1100.040.6980931
GTATCAA41300.040.190221
TATCAAC43500.037.9899832
GGTATCA16200.036.562581
TGGTATC6050.035.2820172
GTGGTAT6000.035.0107961
TTGTATC400.002624664734.4233673
AACGCAG50250.032.53956
CAGAGTA49000.032.31739810
AGAGTAC49150.032.21876511
ATCAACG52450.031.6340583
GCAGAGT51700.031.4286759
CAACGCA52400.031.334245
TATAAGA1450.030.8608174
TATACCT450.004609588530.68413532
TTAGTGG450.00467412830.5970524
CGCAGAG53850.030.173868
Produced by FastQC (version 0.11.5)