FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467555
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT79040.5385828810504547No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61080.41620245919233007No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC56750.3866976024748647No Hit
ATGCTGCACCCGGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA55370.377294207031423No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC48460.3302090892675232No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48270.32891441888038264No Hit
GATCTATGCTGCACCCGGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA47500.323667596785129No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45280.30854039541959244No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43820.29859187560261796No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG43630.2972972052154774No Hit
GTGCAGCCACAGTTCGTAGGATCTCAACCGTGGTCCCTCCGCCGAAAGTG43510.29647951865517813No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC43400.29572997264157047No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC40610.27671876011461244No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39620.2699728459921434No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC39310.2678604890447036No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38320.2611145749222346No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCACC38310.261046434375543No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT37930.25845709360126196No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT37550.25586775282698093No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36210.24673691957030572No Hit
GTGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTGCTAATACC35380.2410812541949024No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG34590.2356981510062655No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA32140.219003717066822No Hit
GAATTCACTCTCACCATCAGCAGCCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTCAGAGTTCC30410.20721540248917417No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30410.20721540248917417No Hit
GGGTCAGAGTTCCCCGCTCACTTTCGGCGGAGGGACCACGGTTGAGATCCTACGAACTGTGGCTGCACCAT29410.20040134782001356No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT29010.1976757259523493No Hit
GCCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTCAGAGTTCCCCGCTCACTTTCGGCGGAGGGA28040.19106609292326354No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27820.18956700089604817No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25710.1751893455441193No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25150.17137347492938937No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24590.1675576043146594No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24550.167285042127893No Hit
GCTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG23880.16271962549955538No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23740.16176565784587288No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23370.15924445761828346No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23340.15904003597820865No Hit
CTCTGACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGGCTGCTGATGGTGAGAGTGAATTCTGTC23260.15849491160467583No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22950.15638255465723602No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22700.15467904098994587No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22380.1524985434958145No Hit
ATTGTCAACAGGGTCAGAGTTCCCCGCTCACTTTCGGCGGAGGGACCACGGTTGAGATCCTACGAACTGTGGCTG21670.14766056468071043No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21300.145139364453121No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC21040.14336771023913925No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTAGGATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCGGGGAACT20970.142890726412298No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACC20810.1418004776652323No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20550.14002882345125056No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20340.13859787197072682No Hit
CCTACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT19980.136144812289829No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19100.13014844418096766No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18990.12939889816736No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18920.12892191434051875No Hit
CACCATCAGCAGCCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTCAGAGTTCCCCGCTCACTTT18650.1270821195798454No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCA18480.12592373028608808No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18340.12496976263240558No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18330.124901622085714No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18270.12449277880556436No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTGCTA18050.12299368677834903No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17780.12115389201767565No Hit
GTTCGTAGGATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCGGGGAACT17610.11999550272391835No Hit
ACCGTGGTCCCTCCGCCGAAAGTGAGCGGGGAACTCTGACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGG17360.11829198905662822No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17330.11808756741655338No Hit
AGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATG17290.11781500522978695No Hit
GTTGAGATCCTACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC17210.11726988085625412No Hit
GTCTCCTACAGACGCAGACACGGAAGATGGAGACTGGGTCATCTGGATGTCGCATCTGGAACCTGGGAACCAGAG17160.11692917812279609No Hit
GTCTCCATCTTCCGTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAG16960.11556636718896396No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC16870.1149531022687395No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTACAGCCTGAAGATTTTG16780.11433983734851505No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT16750.11413541570844024No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16620.11324958860144935No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16320.11120537220070117No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16310.11113723165400956No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16270.11086466946724313No Hit
ACCTTGATGGGACCCCACTTTGCAGACCGGGTGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG16260.11079652892055153No Hit
GACCACGGTTGAGATCCTACGAACTGTGGCTGCACCATCTGTCTTCATCT16250.11072838837385991No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA15960.10875231251980336No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15890.1082753286929621No Hit
GGCTGTAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC15380.10480016081169019No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15190.10350549042454968No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15080.10275594441094202No Hit
GGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATC14920.10166569566387632No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14690.10009846308996938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28350.040.9974141
TAAGGCG5200.036.6876829
TATCAAC32500.035.7562642
CGACCGG1101.0830412E-535.5965970
ATCAACG33200.035.0023653
GACTAAG5500.034.639226
ACGAGAC5500.034.60495822
TCAACGC33600.034.5856744
TCGTATG3050.033.9294240
CAACGCA34350.033.8305325
GGCGAAT5700.033.5680932
GCGAATC4800.033.49701333
CGAGACT5750.033.11055423
ATCTCGT4150.033.10724337
CTTATAC5600.032.7709581
AACGCAG36300.032.1067926
TATACAC6050.031.451553
ACTAAGG6100.031.24378827
CGAGCCC6100.031.19268615
CGCAGAG37300.031.1506798
Produced by FastQC (version 0.11.5)