FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467555
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76230.5194353874301134No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC57400.391126738009819No Hit
ATGCTGCACCCGGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA53420.3640068004265598No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50290.3426788093120871No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48000.32707462411970933No Hit
GATCTATGCTGCACCCGGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA47810.3257799537325688No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45250.30833597377951766No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45230.3081996926861344No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44250.30152191911035703No Hit
GTGCAGCCACAGTTCGTAGGATCTCAACCGTGGTCCCTCCGCCGAAAGTG43980.29968212434968367No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC43320.29518484826803765No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT42660.29068757218639163No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC41040.2796488036223515No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40120.2733798733267237No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37230.2536872553328495No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCACC36500.24871299542436226No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC36060.24571481136993162No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG35620.24271662731550095No Hit
GTGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTGCTAATACC35100.23917331888753743No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT33790.23024690727093702No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33740.229906204537479No Hit
GGGTCAGAGTTCCCCGCTCACTTTCGGCGGAGGGACCACGGTTGAGATCCTACGAACTGTGGCTGCACCAT31820.21682321957269066No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG31330.21348433278480192No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31000.21123569474397896No Hit
GAATTCACTCTCACCATCAGCAGCCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTCAGAGTTCC29470.20081019110016318No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT29420.2004694883667052No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28750.19590407173836755No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA28540.19447312025784383No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27470.18718208176184198No Hit
GCCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTCAGAGTTCCCCGCTCACTTTCGGCGGAGGGA27070.18445645989417775No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26020.17730170249155908No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25990.17709728085148427No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25470.17355397242352075No Hit
CTCTGACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGGCTGCTGATGGTGAGAGTGAATTCTGTC25230.1719185993029222No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24280.16544524736721963No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23310.15883561433813384No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22630.15420205716310462No Hit
GCTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG22370.15243040294912286No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22200.15127201365536555No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22040.15018176490829988No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21560.14691101866710277No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21440.1460933321068035No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA21390.14575262937334546No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21150.1441172562527469No Hit
ATTGTCAACAGGGTCAGAGTTCCCCGCTCACTTTCGGCGGAGGGACCACGGTTGAGATCCTACGAACTGTGGCTG21040.14336771023913925No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20980.14295886695898963No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20480.13955183962440929No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20350.13866601251741842No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19500.1328740660486319No Hit
CCTACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT19480.1327377849552487No Hit
GGCTGTAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC19300.1315112551147998No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19090.13008030363427606No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTAGGATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCGGGGAACT18760.12783166559345305No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18610.12680955739307898No Hit
CACCATCAGCAGCCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTCAGAGTTCCCCGCTCACTTT18580.12660513575300414No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC18440.12565116809932164No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCA18400.12537860591255523No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18390.12531046536586363No Hit
GTTCGTAGGATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCGGGGAACT18350.1250379031790972No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTGCTA18290.12462905989894758No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACC18120.12347067060519026No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18090.12326624896511545No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17990.12258484349819938No Hit
GTTGAGATCCTACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC17580.11979108108384354No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17270.11767872413640373No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17220.11733802140294572No Hit
GTCTCCTACAGACGCAGACACGGAAGATGGAGACTGGGTCATCTGGATGTCGCATCTGGAACCTGGGAACCAGAG17060.11624777265588002No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT16960.11556636718896396No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16680.11365843188159898No Hit
ACCGTGGTCCCTCCGCCGAAAGTGAGCGGGGAACTCTGACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGG16390.1116823560275424No Hit
GTCTCCATCTTCCGTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAG15970.10882045306649495No Hit
GACCACGGTTGAGATCCTACGAACTGTGGCTGCACCATCTGTCTTCATCT15770.10745764213266283No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC15640.10657181502567196No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTACAGCCTGAAGATTTTG15220.1037099120646245No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15210.1036417715179329No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15150.10323292823778325No Hit
ACCTTGATGGGACCCCACTTTGCAGACCGGGTGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG14890.1014612740238015No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14890.1014612740238015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)