FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378346
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63010.45714211090684054No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT45920.3331529238667214No Hit
GTCTTCTACAGCTCCACCAGTAGGAACTACTTAGCCTGGTACCAACACAA45530.3303234456370171No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44940.32604295293054136No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38480.279175185330824No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38080.27627315637728117No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36180.26248851884795255No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35190.25530599718793395No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35130.2548706928449025No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34950.2535647798158082No Hit
ACTTAGGAGGCTGCCCTGGTTTGTGTTGGTACCAGGCTAAGTAGTTCCTACTGGTGGAGCTGTAGAAGACACT34630.2512431566529739No Hit
GTACCAACACAAACCAGGGCAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA33320.241739011830121No Hit
CTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAACTGTGGCTGCACCA32670.2370232147806139No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGTTTGGTCCCCTGGCCAAAAGTG31230.22657591054785955No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC30650.2223679685652224No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30170.21888553382097092No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTCTTCTA29280.21242851939933804No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27940.20270672240496942No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCAC27340.19835367897465514No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26920.1953065485734351No Hit
ATATCATAGTAGTCCGAACACTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAACTGTGGCTGCACCAT26770.19421828771585653No Hit
ACACAAACCAGGGCAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT26430.1917515631053451No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25310.18362588203542507No Hit
GTGTATTACTGTGCGAGAGGCTTCCGTAGTAGTACCAGCGCAGACGCTTT25280.18340822986390937No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24910.18072385308188219No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24770.1797081429481422No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT24720.17934538932894933No Hit
GTAATACACGGCCGTGTCCCCGACTCTCAGGCTGTTCATTTGCAGATACA24170.1753550995178279No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24000.17412173721257218No Hit
ATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAGCAGCC23770.17245307056428502No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC23360.1694784908869036No Hit
GGCCAAAAGTGTTCGGACTACTATGATATTGCTGACAGTAATAAACTGCC22630.16418228804668786No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21990.15953904172101926No Hit
AACCAGGGCAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA21920.15903118665414925No Hit
GTTTATTACTGTCAGCAATATCATAGTAGTCCGAACACTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAA21820.15830567941576354No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21510.15605660697676782No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21500.15598405625292924No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20720.15032509979352066No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20210.14662501287775348No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19960.1448112447817892No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC19920.1445210418864349No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19600.1421994187236006No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19320.14016799845612057No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTCTTCTACAGCTCCAC19100.13857188253167202No Hit
GTAGAAGACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA18930.1373385202264163No Hit
CTATGATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCC18830.13661301298803058No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTCTTCTACAGCTCCACCAGTAGGAACTA18800.13639536081651488No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18690.13559730285429059No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18650.1353070999589363No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18550.13458159272055056No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18500.13421883910135773No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17990.13051875218559056No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGCCCTGGTTTGTGTTGGTA17960.13030110001407486No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17830.1293579406041734No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17550.1273265203366934No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA17530.12718141888901627No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17400.12623825947911482No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG17190.12471469427850482No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16930.12282837545870195No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16400.11898318709525765No Hit
ACTTAGCCTGGTACCAACACAAACCAGGGCAGCCTCCTAAGTTGCTCATT16290.11818512913303336No Hit
GTGTTGGTACCAGGCTAAGTAGTTCCTACTGGTGGAGCTGTAGAAGACAC16160.11724196972313192No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16040.11637136103706906No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT16010.11615370886555335No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15850.11499289728413621No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15750.11426739004575048No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15610.11325167991201048No Hit
GGTTTGTGTTGGTACCAGGCTAAGTAGTTCCTACTGGTGGAGCTGTAGAA15570.1129614770166562No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGTTTGGTCC15520.11259872339746335No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15340.11129281036836905No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAGCAGC15220.11042220168230618No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA15090.10947904227240476No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14800.10737507128108618No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATCATAGTAGT14600.10592405680431474No Hit
ACTATGATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGC14530.10541620173744473No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14530.10541620173744473No Hit
CTACTTAGCCTGGTACCAACACAAACCAGGGCAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATC14240.10331223074612615No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14180.10287692640309472No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14170.10280437567925615No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14170.10280437567925615No Hit
GAACTACTTAGCCTGGTACCAACACAAACCAGGGCAGCCTCCTAAGTTGC14130.10251417278390187No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14130.10251417278390187No Hit
TATCATAGTAGTCCGAACACTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAACTGTGGCTGCACCAT14030.10178866554551615No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA13960.10128081047864615No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13830.1003376510687447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3050.044.0338442
GTGGTAT3100.042.215821
GGTATCA8550.041.4881861
CCGTTAT350.00129446839.7543649
GTATCAA20600.037.7828371
TTAGACC400.002449298734.9130255
CAACGTT601.9587053E-434.2021869
TATCAAC24200.032.5868152
TCGTATG1305.456968E-1231.96169340
CTCGTAT1305.456968E-1231.95120239
ACAATGG14500.031.623370
ATCAACG26000.030.4643613
TCAACGC26300.030.24784
AACGCAG26750.029.8676996
CAACGCA26850.029.756465
AACGTTG900.00731396627.91706870
GTTAGAC500.007329133327.90680754
CGCAGAG28700.027.8353318
AGAGTAC28550.027.37554611
CAGCGCT6200.027.2171084
Produced by FastQC (version 0.11.5)