FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378346
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63880.46345402388079626No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT41970.3044953879504856No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41090.29811092425269126No Hit
GTCTTCTACAGCTCCACCAGTAGGAACTACTTAGCCTGGTACCAACACAA39550.28693811278155124No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38590.27997324329304835No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37580.2726456201853526No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36950.26807492458352256No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36740.2665513593829126No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36280.26321402608633826No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGTTTGGTCCCCTGGCCAAAAGTG35080.25450793922570963No Hit
ACTTAGGAGGCTGCCCTGGTTTGTGTTGGTACCAGGCTAAGTAGTTCCTACTGGTGGAGCTGTAGAAGACACT32280.23419373655090958No Hit
CTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAACTGTGGCTGCACCA31560.2289700844345324No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30050.21801492513490806No Hit
GTACCAACACAAACCAGGGCAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA29260.21228341795166092No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29050.21075985275105089No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28000.20314202674800086No Hit
GGCCAAAAGTGTTCGGACTACTATGATATTGCTGACAGTAATAAACTGCC27650.20060275141365086No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27500.19951449055607226No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27250.19770072246010797No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26520.19240451961989224No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26370.19131625876231367No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25620.1858749544744208No Hit
ATATCATAGTAGTCCGAACACTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAACTGTGGCTGCACCAT25590.18565730230290506No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCAC25370.1840611863784565No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTCTTCTA24600.1784747806428865No Hit
GTGTATTACTGTGCGAGAGGCTTCCGTAGTAGTACCAGCGCAGACGCTTT24320.17644336037540648No Hit
ACACAAACCAGGGCAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT23800.17267072273580072No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23270.16882553437235645No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23220.16846278075316357No Hit
GTAATACACGGCCGTGTCCCCGACTCTCAGGCTGTTCATTTGCAGATACA23040.1671568677240693No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22120.16048220113092068No Hit
CTATGATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCC22030.15982924461637354No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21950.15924883882566496No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21500.15598405625292924No Hit
GTTTATTACTGTCAGCAATATCATAGTAGTCCGAACACTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAA21190.15373498381393352No Hit
ATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAGCAGCC21000.15235652006100064No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20780.15076040413655206No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA20580.14930938965978063No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20570.14923683893594206No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19840.14394063609572633No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19800.14365043320037205No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19640.14248962161895487No Hit
AACCAGGGCAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA18790.1363228100926763No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18160.13175211449084628No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18060.13102660725246054No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC18050.130954056528622No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17930.13008344784255912No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17900.12986579567104342No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17710.12848733191811054No Hit
ACTATGATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGC17680.12826967974659484No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17590.12761672323204767No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17330.1257304044122448No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17270.12529510006921338No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA17270.12529510006921338No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16490.1196361436098048No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16420.11912828854293481No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16300.11825767985687193No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16290.11818512913303336No Hit
GTGTTGGTACCAGGCTAAGTAGTTCCTACTGGTGGAGCTGTAGAAGACAC16170.11731452044697051No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTCTTCTACAGCTCCAC16010.11615370886555335No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGCCCTGGTTTGTGTTGGTA15730.11412228859807333No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15720.11404973787423477No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC15580.11303402774049476No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15460.1121634190544319No Hit
GGTTTGTGTTGGTACCAGGCTAAGTAGTTCCTACTGGTGGAGCTGTAGAA15380.11158301326372334No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15300.11100260747301474No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15270.11078495530149905No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15240.11056730312998334No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15050.10918883937705046No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14970.10860843358634188No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTCTTCTACAGCTCCACCAGTAGGAACTA14960.10853588286250332No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14950.10846333213866476No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14780.10722996983340903No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14710.10672211476653902No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14190.10294947712693331No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13970.10135336120248471No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATCATAGTAGT13970.10135336120248471No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13900.1008455061356147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8400.046.2673451
GTATCAA21350.041.239891
TATCAAC24950.035.2804832
ATCAACG26050.033.922713
TCAACGC26250.033.664254
CAACGCA26300.033.600255
AACGCAG26400.033.343946
GTGGTAT2300.032.898061
TAAGACT1900.032.5761539
AGAGTAC27400.031.74936711
TCTATAC450.00400577531.57801266
ACAATGG13000.031.09010970
CGCAGAG28200.030.9717318
CAGAGTA28400.030.63143210
GTGCTAA802.8295926E-630.0942481
TGGTATC2550.029.6652242
GCAGAGT29400.029.5906229
ACGCAGA30400.028.956587
TATAAGA2000.027.5077532
TGTTAGA8950.027.23865170
Produced by FastQC (version 0.11.5)