FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686560
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACA63260.375083009202163No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC45720.2710843373493976No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT44670.2648586471871739No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT44600.26444360117635896No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44040.26112323308983965No Hit
GTGTATTACTGTGCACGAGCAGTAGGAGTAGCTCGTCCGTTCTCCCTCCA39310.2330779812162034No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC36790.2181363248268665No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32230.1910990418366379No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATA31470.18659282800493313No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30090.1784104923631534No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA28800.17076178730670713No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG28200.16720425007115075No Hit
CTCCTGTGCAGCCTCTGGATTCACCCTCAGAAACTTCGACATACACTGGGTCCGCCAAGGTACAGGGAAAGGT27510.1631130822502609No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG27260.16163077506877904No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27240.1615121904942605No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26980.15997059102551942No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26590.15765819182240773No Hit
GTCTATTACTGTCAGCGCTTTGGTGGGTCACCTCCGTACACTTTTGGCCA26300.1559387154918888No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25970.1539820700123328No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT25930.1537449008632957No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT25770.15279622426714734No Hit
GATCAGGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGG25390.15054311735129494No Hit
CTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCACCTACTTAGCCTGGTACC24670.14627407266862727No Hit
GTACAGGGAAAGGTCTGGAGTGGGTCTCAAGTAGTGATACTTCTGGTGAC24640.14609619580684946No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC24480.14514751921070107No Hit
GGTCTGGAGTGGGTCTCAAGTAGTGATACTTCTGGTGACACATACTATCT24310.14413955032729342No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24160.14325016601840432No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23990.14224219713499667No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT23560.13969262878284794No Hit
GTAATAGACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG23540.13957404420832936No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC23510.13939616734655155No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT23480.13921829048477374No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23390.1386846598994403No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23320.13826961388862538No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT23100.13696518356892134No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22440.13305189260980932No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG22260.13198463143914238No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA22000.1304430319704013No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21820.12937577079973436No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGGTGACCCACCAAAGCG21710.12872355563988236No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21620.1281899250545489No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT21370.12670761787306706No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA21030.12469168010625178No Hit
CTATTACTGTCAGCGCTTTGGTGGGTCACCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA21010.12457309553173324No Hit
GTCCCAGACCCACTGCCGCTGAACCTGTCTGGGATGCCAGTGGCCCTGCT20980.1243952186699554No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT20500.12154918888151028No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG20020.11870315909306517No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA20010.1186438668058059No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA19760.11716155962432408No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG19030.11283322265439712No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT19030.11283322265439712No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCA19020.11277393036713784No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA18970.11247746893084148No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAG18540.10992790057869273No Hit
CAGTAATAGACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG18200.10791196281187744No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA17960.10648894791765488No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTTCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC17800.10554027132150649No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17800.10554027132150649No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG17680.10482876387439523No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCA17640.10459159472535813No Hit
ATCTAGGCTCCGTGAAGGGCCGATTCACCATCTCCAGAGAAAATGTCAAG17570.10417654871454321No Hit
GGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC17400.10316857983113557No Hit
GCCTTGGGATTCCCAAGTGTTTGTTTTCGCTGATCAGGACTGAACACACA17250.10227919552224646No Hit
GGGTGTTAGCAGCACCTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGG17240.1022199032349872No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC17230.10216061094772792No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA17210.10204202637320936No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGTGGAGCCCCAGCCTTGGGATTCCCAAGTGTTTGTTTTCGCTGAT17090.1013305189260981No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17010.1008561806280239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12900.038.0499271
GTATCAA28050.036.466231
AACCGTA400.00259374334.50794635
TATCAAC32850.031.0250632
AACGCAG33550.030.6864076
ATCAACG33850.030.2081013
TCAACGC34200.029.8980674
CAACGCA34650.029.7113555
CGCAGAG36350.028.4170748
AGAGTAC36650.027.71628811
GCAGAGT37450.027.5823969
CGATTGT950.00844529527.10673370
CCGTTAA858.279972E-526.42940568
AATACAC23050.025.9026473
TAATACA22900.025.7741622
CAGAGTA39850.025.7489910
GTAATAC22850.025.537021
TACTCGA700.001099879325.47255566
TATAAGA2350.024.824052
ACGCAGA41400.024.7849567
Produced by FastQC (version 0.11.5)