FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470343
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65410.4448621852180069No Hit
GAGTTACAGTAATCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT53590.3644727794807062No Hit
GTACATTTAGTATGCAAAGTGGGGTCCCACCAAGGTTCAGTGGCAGTGGA45400.30877149073379473No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44000.29924990291380993No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41950.2853075778916892No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40360.27449377458184926No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG40130.2729295137257089No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38360.26089150626758517No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC37180.2528661679621694No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36400.24756128331960636No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35940.24443276160732563No Hit
CTGTAACTCTGTTGACAGTAGTAAATTGCAGAATCCTCAGGTTGCAGACTGCTGATGGCGAGACTGAAATCTGTC33850.23021839121891968No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32680.22226106425507516No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG31790.21620805485522768No Hit
GTCTCCATCCTCCCTGTCTGCATTTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGAGCATTAGTA31420.21369163521708878No Hit
CTAAATGTACCATGGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAAGTACTAATG30050.2043740814218179No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29750.2023337411746783No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28650.19485249360183304No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC28180.19165596054798098No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT28140.1913839151816957No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27440.18662312127170327No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27110.18437874699984969No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25760.17519721588772144No Hit
GTCTGCAACCTGAGGATTCTGCAATTTACTACTGTCAACAGAGTTACAGTAATCCGTGGACGTTCGGCCAAGGGA25680.17465312515515088No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25610.17417704576415163No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25290.17200068283386938No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24930.16955227453730184No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTCCACGGATTA23610.16057477744988755No Hit
ACAGTAATCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGA23170.15758227842074946No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23070.15690216500503623No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22780.15492983609946795No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22300.15166529170404455No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC21970.149420917432191No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21690.14751659986819401No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20750.1411235337604899No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20700.1407834770526333No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20200.13738290997406727No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20150.13704285326621068No Hit
GATTACTGTAACTCTGTTGACAGTAGTAAATTGCAGAATCCTCAGGTTGCAGACTGCTGATGGCGAGACTGAAAT19910.135410581068499No Hit
ATTTACTACTGTCAACAGAGTTACAGTAATCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA19630.133506263504502No Hit
CTCCTGATCCATGGTACATTTAGTATGCAAAGTGGGGTCCCACCAAGGTT19180.13044575313379259No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC19020.12935757166865144No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC18870.12833740154508166No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT18110.1231685395856613No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17640.11997200653180923No Hit
GGTTGCAGACTGCTGATGGCGAGACTGAAATCTGTCCCAGCTCCACTGCC17610.11976797250709528No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17570.11949592714081No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGAGCATTAGTACTTATTTAAATTGGTATCAGCAGAAA17400.11833973433409756No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGATTACTGTAACTCTGTTGACAG17010.11568729201281605No Hit
GTAGTAAATTGCAGAATCCTCAGGTTGCAGACTGCTGATGGCGAGACTGAAATCTGTCCCAGCTCCACTGCCA16990.11555126932967341No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16810.11432706518138966No Hit
GTTCAGTGGCAGTGGAGCTGGGACAGATTTCAGTCTCGCCATCAGCAGTCTGCAACCTGAGGATTCTGCAATTTA16790.114191042498247No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16780.11412303115667569No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16710.11364695176567645No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATTTGTAGGAGACAGAGTCACCATCA16580.11276280432524928No Hit
GTGGGGTCCCACCAAGGTTCAGTGGCAGTGGAGCTGGGACAGATTTCAGT16390.1114705888353942No Hit
GATTTCAGTCTCGCCATCAGCAGTCTGCAACCTGAGGATTCTGCAATTTACTACTGTCAACAGAGTTACAGTAAT15960.10854610114782741No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15770.10725388565797232No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15720.10691382895011572No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15720.10691382895011572No Hit
ATGGTACATTTAGTATGCAAAGTGGGGTCCCACCAAGGTTCAGTGGCAGTGGAGCTGGGACAGATTTCAGTCTCG15460.10514553406926139No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15430.10494150004454743No Hit
GCATTAGTACTTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT15370.10453343199511951No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15200.10337723918840706No Hit
AGTCAGAGCATTAGTACTTATTTAAATTGGTATCAGCAGAAACCAGGGAA14940.10160894430755271No Hit
GAGCATTAGTACTTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC14940.10160894430755271No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14750.10031672881769763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52300.040.1513181
GCCTAAT700.001868885636.8892370
TATCAAC57300.036.582952
GTGGTAT6200.036.466431
AACGCAG61500.034.2563446
ATCAACG61600.034.029273
CAACGCA62400.033.7062155
TCAACGC63250.033.2498554
GTTATTA1002.7509528E-432.27807270
CGCAGAG66200.031.722918
AGAGTAC65050.031.65174911
GCAGAGT66750.031.4615239
ACGCAGA68350.030.9756557
CAGAGTA68650.030.14164410
TGTTAGA14000.029.05026870
TATAAGA3900.027.2256952
AGTACGG57050.026.96165313
GAGTACG58250.026.58265112
TCTATAC1101.2280561E-624.9103163
ATAAGAG4100.024.2267993
Produced by FastQC (version 0.11.5)