FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847926
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACTA350.00539803855.96178470
GGGTAAG252.5808054E-455.139221
TAAGAAC252.5823098E-455.132723
TAACAAT200.007390490351.6930241
TATACGG306.34855E-445.9439352
TATACAC1000.044.7953343
GTGGTAT3250.042.4147871
GTATCAA26150.040.590061
AGGGGGG1451.0713848E-940.52404870
GGTATCA10250.039.0009161
GTATACG451.0078601E-438.2911261
TGGTATC3650.037.7621352
CTTATAC1051.6370905E-1136.103061
TATCAAC29400.036.0988042
TTATACA1051.6370905E-1136.0988042
ATACACA1051.6370905E-1136.0988044
CAGAGTA32800.031.4076610
AACGCAG33650.031.3347196
AGAGTAC32900.031.31219511
CAACGCA34650.030.629295
Produced by FastQC (version 0.11.5)