FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116155
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA72470.6492825817202807No Hit
GTCTGTAGACCTTGCATGCAGTAATAAATTCCCACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT63610.5699029256689259No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52550.47081274554161384No Hit
GTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTT51050.45737375185346124No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38100.3413504396790768No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34190.30631946279862565No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29930.26815272072427215No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29360.26304590312277415No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC28990.25973095134636315No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28800.2580286788125305No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27580.2470982972794997No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27100.2427978192992909No Hit
GACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTGGGAATTTATTACTGCATGCAAGGTCTACAGACT27100.2427978192992909No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT26970.2416331065129843No Hit
GTCCCTGACAGGTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAA25720.23043394510619042No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24920.22326648180584235No Hit
GTGTAAAGTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCG24600.22039949648570314No Hit
CCCCTGGAGATCCGGCCGACATCTCCTGTAGGTCTAGTCAGAGCCTCTTG23920.2143071526804073No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG23560.21108179419525064No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22430.200957752283509No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGATCCGGCCGACATCTCCT21930.19647808772079145No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAAGCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA21910.19629890113828274No Hit
ATCCATTACTATGCAAGAGGCTCTGACTAGACCTACAGGAGATGTCGGCC21820.1954925615169936No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20660.1850997397314889No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20020.17936576909121046No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCGAGGAGT19700.17649878377107123No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19670.17623000389730817No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTTATCGGGCC19560.17524447769351031No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC19460.17434854478096679No Hit
GGTCTAGTCAGAGCCTCTTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC18970.1699584735095036No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18830.1687041674319427No Hit
CTCCACAACTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCT18060.16180548400535769No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT17690.1584905322289467No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC17510.1568778529863684No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA17260.1546380207050096No Hit
CTGTAGACCTTGCATGCAGTAATAAATTCCCACATCCTCAGCCTCCACTC16550.1482768970259507No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16120.1444243855020136No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16030.14361804588072447No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15860.1420949599294005No Hit
CTCCCTGCCCGTCACCCCTGGAGATCCGGCCGACATCTCCTGTAGGTCTA15720.14084065385183958No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15600.13976553435678737No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15510.13895919473549823No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15500.13886960144424387No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14990.13430034359027196No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14960.1340315637165089No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14940.1338523771340002No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14810.13268766434769366No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCTGA14780.13241888447393058No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14630.13107498510511534No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14460.12955189915379137No Hit
CCTGTAGGTCTAGTCAGAGCCTCTTGCATAGTAATGGATACACCTATTTG14460.12955189915379137No Hit
GGTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14180.12704328699866954No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13920.12471386142605641No Hit
CTCCTGTAGGTCTAGTCAGAGCCTCTTGCATAGTAATGGATACACCTATT13910.12462426813480205No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13380.11987582369832146No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13270.1188902974945236No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13070.11709843166943659No Hit
GGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTTAT12910.11566493900936699No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT12880.11539615913560393No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCATTACTATGC12780.11450022622306041No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12650.11333551343675385No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12500.1119916140679386No Hit
GTCTCCACAACTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCTGACAGGTTCACTGGCAGTGGATC12490.11190202077668425No Hit
TCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACC12380.11091649457288638No Hit
GTGGAGGCTGAGGATGTGGGAATTTATTACTGCATGCAAGGTCTACAGAC12370.11082690128163203No Hit
GTAATAAATTCCCACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA12350.11064771469912332No Hit
CACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTTATCGGGC12320.11037893482536028No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCATTACTATGCAAGAGGCTCTGA12130.10867666229152761No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC12000.10751194950522104No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCTGACAGGTT11980.10733276292271235No Hit
GAGTGGAGGCTGAGGATGTGGGAATTTATTACTGCATGCAAGGTCTACAGACTCCTCGGACTTTCGGCGGAGGGA11910.10670560988393189No Hit
CTCTTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT11830.10598886355389708No Hit
GTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAAAATCAGCAGAG11720.10500333735009922No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11610.10401781114630136No Hit
AGTCAGAGCCTCTTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA11480.1028530983599948No Hit
GTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCGGAGGCCC11450.10258431848623176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1000.041.5535140
GTATCAA20850.040.0900841
GGTATCA8850.037.7020721
CTTATAC1650.035.440661
CGTATGC1102.7284841E-1134.63886641
TATCAAC24350.034.6086352
GTCTAGA501.89396E-434.3982851
GTGGTAT3300.034.3982851
TGGTATC3350.033.8833582
CTGTCTT26000.033.6205770
GCTAATC1253.6379788E-1233.11583733
ATCAACG26100.032.0245553
TCAACGC26450.031.6007864
AACGCAG26750.031.2463866
CAACGCA26850.031.1300135
TTAACGG1001.43263605E-831.08800735
CGAGACT1700.030.35414523
ATAATAC802.8268296E-630.0971493
GTAGTTT1702.7220267E-729.59406970
TTATACA2000.029.2372322
Produced by FastQC (version 0.11.5)