FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542765
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACAGAACTCAGGCTGCTGTCATAGGT40700.2638120517382751No Hit
GTAATACACAGCCGAGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA39010.2528576938159733No Hit
CCTCATAGTTGCTCACTTCAATCGGCCCTCAGGGGTCTCTGACCGATTCTCCGGCTCCAAGTCTGGCACCTCAGC35580.23062488454171567No Hit
GTGTATTACTGTGCGAGAGATTCTCCGACGTATGGTTCGGGGACTTATTC32710.21202192167958178No Hit
GACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACAGAACTCAGG31920.20690124549105016No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30300.19640061837026376No Hit
CTCCTGCACTGGGGACAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC29450.19089103006614747No Hit
GTCATAGGTCTGGCAGTAATAGTCAGCCTCATCCTCAGCCTGGAGCCCAG24510.15887059921634208No Hit
ACTATGAGGAGTGCGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTTGGAG22650.14681432363321698No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21480.1392305373793157No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20670.13398022381892252No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20630.1337209490751994No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTC19780.12821136077108308No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18860.12224804166545132No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18750.12153503612021273No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18590.12049793714532026No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18000.11667363467540422No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG17680.11459943672561927No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17030.11038622214011855No Hit
ACCTATGACAGCAGCCTGAGTTCTGTGATTTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCCAAGG16750.10857129893405672No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCGGAGAATCGGTC16460.1066915570420641No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16390.10623782624054863No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAG200.0074802451.537451
TACTGGG12400.038.5819270
GGTATCA11200.038.0395471
GTATCAA24000.033.3561821
CTTATAC2150.031.961211
TATACAC2250.030.5426943
TATCAAC26400.030.3247872
GTGGTAT4950.029.8466051
TCGTATG2100.028.03196140
ATCAACG28750.027.9664823
TCAACGC28900.027.7024354
AACGCAG29100.027.6301166
GTAATAC11200.027.6093481
CAACGCA29550.027.0930735
TAATACA11400.026.5230582
ACTACTG10850.026.08699868
TTGACTA10200.026.04357365
TGGTATC5550.026.001722
ATACACA14700.025.9457074
AGAGTAC30800.025.76204511
Produced by FastQC (version 0.11.5)