FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542765
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGAGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA34470.22343001040339908No Hit
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACAGAACTCAGGCTGCTGTCATAGGT34080.22090208165209865No Hit
CCTCATAGTTGCTCACTTCAATCGGCCCTCAGGGGTCTCTGACCGATTCTCCGGCTCCAAGTCTGGCACCTCAGC33940.21999462004906772No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33540.21740187261183652No Hit
GTGTATTACTGTGCGAGAGATTCTCCGACGTATGGTTCGGGGACTTATTC31520.20430849805381895No Hit
CTCCTGCACTGGGGACAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC29460.19095584875207824No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29300.18991874977718576No Hit
GACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACAGAACTCAGG24010.15562966491980307No Hit
GTCTATGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTGCACTGGGGACAG22090.14318447722109331No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22040.1428603837914394No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21250.13773970760290777No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21020.13624887782649983No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19750.12801690471329075No Hit
GTCATAGGTCTGGCAGTAATAGTCAGCCTCATCCTCAGCCTGGAGCCCAG19570.1268501683665367No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19420.125877888077575No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17520.11356233775072677No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG17230.11168259585873416No Hit
GTCCTGGGCCCAGTCTATGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTG17050.1105158595119801No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTC16600.10759901864509501No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16110.10442290303448677No Hit
ACCTATGACAGCAGCCTGAGTTCTGTGATTTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCCAAGG15980.10358026011738665No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15830.10260797982842493No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15610.10118196873794777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)