FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207663
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATT200.006282825553.8609266
ATGATAG200.00703848752.3362747
ACTAATC200.00761252551.30875422
CTTATAC1500.047.8901521
TTATACA1500.047.874292
GTGGTAT4200.046.424131
TATACAC1650.045.5964473
TCTTATA405.270415E-542.74492
CTAATCT1550.042.2341234
ATACACA1750.041.0368044
CGCTAAT1700.040.4532832
TAATCTC1650.039.70594835
GGTATCA14500.039.3973161
TGTGTAT801.7898856E-938.4688212
GTATCAA32000.037.9486731
TGGTATC5250.037.1272
TATCAAC34700.034.8857542
CTCTTAT501.9570506E-434.2072531
TCGTATG2150.033.8222240
TCGCTAA2300.032.86017631
Produced by FastQC (version 0.11.5)