FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207663
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17430.14432834325469937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATA150.002439068968.396534
GTGGTAT5550.041.914461
GTCTAAA350.001405027639.0966841
GTATCAA35000.038.607971
GGTATCA15300.035.7747421
TATCAAC37950.035.5965042
TGGTATC6550.035.5050162
ATAAGAG1101.1623342E-931.0906183
TACTGTG450.004822804630.4022337
TAACCAT450.004823781530.4009765
AGAGTAC43000.030.22547711
TAGCACA802.9575021E-629.9234834
CAGAGTA43350.029.90254610
GTATAAG1151.8644641E-929.7474751
TATAAGA1151.87174E-929.7388532
AACGCAG45750.029.5288146
GCAGAGT45700.029.2629819
CAACGCA47000.028.8890135
ATCAACG47350.028.6020573
GAGTAAG605.6972785E-428.5079981
Produced by FastQC (version 0.11.5)