FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376418
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAAATATATTGTGCTCTTCGAATTGTCTCTTGA119690.5036571848891904No Hit
GTCTATTACTGTGCGAAGGATAACAGTCTGTCGATGAGTTGGCTCGACCC94360.3970681925486173No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73600.30970982377679346No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG61700.2596344582476652No Hit
GTAATATACTGCAACATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCGGTCCCAGATCCACTGCCA58180.24482224928442722No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54790.2305570821294907No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC50950.21439830871504928No Hit
GTATATTACTGTCAGAAGTATAAGAATGGCCCTCCAGGTTTCGGCCCTGGGACCAAAGTGGATCTCAAACGAA50790.21372502648944755No Hit
CTCCTGTGTAGTGTCTGGATCCTCCTTCAGTCGTTATGGCATACATTGGGTCCGCCAGGCTCCAGGCGAGGGG49470.20817044812823332No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47130.19832369557880813No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG46760.19676673043210413No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT46460.19550432625910089No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45770.19260079666119342No Hit
GTATAAGAATGGCCCTCCAGGTTTCGGCCCTGGGACCAAAGTGGATCTCAAACGAACTGTGGCTGCACCAT45700.1923062356874927No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG44920.1890239848376843No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA44600.18767742038648083No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44560.1875090998300804No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43140.18153372007786509No Hit
CTGTTATCCTTCGCACAGTAATAGACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAAATATATTGTGCT42950.18073419743496302No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42330.17812522881075635No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG41180.17328601281424394No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC40600.1708453647464377No Hit
CCTGTGTAGTGTCTGGATCCTCCTTCAGTCGTTATGGCATACATTGGGTC40100.16874135779143232No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC39530.16634278986272616No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG39320.1654591069416239No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG38220.16083029164061205No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC37720.15872628468560665No Hit
GTGCAGCCACAGTTCGTTTGAGATCCACTTTGGTCCCAGGGCCGAAACCT36830.15498115230569706No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36660.15426578994099524No Hit
TTCTTATACTTCTGACAGTAATATACTGCAACATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCGGTC36360.153003385767992No Hit
GTCTCCATCCTCCCTGTCTGCATCAGTGGGAGACAGAGTCACCATCACTT36030.15161474117768844No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACAAGATGTGACAT35010.14732256698947743No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGTGTTTATTTAGCCTGGTATCAGCAGAAG34270.14420863669606945No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG33940.1428199921057659No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG33900.14265167154936548No Hit
CTCCTGATCTATGCCGCATCCACTCTGCAAACAGGGGTCCCATCTCGGTT33640.14155758793276266No Hit
GGATAACAGTCTGTCGATGAGTTGGCTCGACCCCTGGGGCCAGGGAACCC33450.14075806528986062No Hit
GTCTGCATCAGTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG33320.14021102348155923No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33280.14004270292515877No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC33250.13991646250785847No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32880.1383594973611545No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC32240.13566636845874758No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT32190.13545596776324703No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA32070.13495100609404576No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31630.133099479973641No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT31390.13208955663523841No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31220.1313741942705366No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC30690.12914394689823086No Hit
CAGTAATATACTGCAACATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCGGTCCCAGATCCACTGCCA30680.12910186675913077No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG30650.12897562634183043No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCC30270.12737658105602634No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC30230.1272082604996259No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG29470.12401016992801771No Hit
ATATTACTGTCAGAAGTATAAGAATGGCCCTCCAGGTTTCGGCCCTGGGA28940.12177992255571199No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT28790.12114872046921038No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG28720.12085415949550964No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA28650.12055959852180888No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27050.11382677626579163No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGT26150.11003956374678193No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26030.10953460207758063No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26020.10949252193848051No Hit
GTCCAGTGTCAGGTGAAGTTGCTGGAGTCTGGGGGAGGCGTGGTCCAGCC25920.10907172054747945No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT25390.10684147317517373No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25260.10629443136687233No Hit
CTGCAAACAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACCGATTTCACTCTCAGCATCAGCAGCCTG25210.1060840306713718No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25190.10599987039317157No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGGTGAGGAGACGATGACCAGGGTTCCCTGG25010.10524242788936963No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACAAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC24920.10486370663746866No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT24790.10431666482916727No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24560.10334882162986478No Hit
CCTTGGGGCTGGTCGGGGATGGTGAGGAGACGATGACCAGGGTTCCCTGG24130.10153937564856014No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG24020.10107649411845897No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23960.10082401328385832No Hit
CCCTGAGACTCTCCTGTGTAGTGTCTGGATCCTCCTTCAGTCGTTATGGC23910.10061361258835778No Hit
GATCAGGAGTTTGGGAACTTTCCCTGGCTTCTGCTGATACCAGGCTAAAT23890.10052945231015756No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23790.10010865091915648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6000.044.179941
CGTATGC1300.042.6242541
GTATCAA41950.040.7859041
TATCAAC47250.036.1350752
TCGTATG1650.035.6650540
ATCAACG50550.034.0484923
TCAACGC50950.033.9838754
CTCTTGA30800.033.97504469
AACGCAG51250.033.852116
CAACGCA51300.033.8191155
ACGCAGA54600.031.832127
GTGTATA2950.031.5084741
GACGTAG2200.031.30354526
CGAATTG29750.030.76227661
CGCAGAG56150.030.7075848
TGTTAGA13200.030.68233970
AGAGTAC54500.030.50059711
ATTGTCT30300.030.30783764
CAGAGTA55150.030.20351810
GAATTGT30450.030.2000362
Produced by FastQC (version 0.11.5)