FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717574
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34200.47660589709214657No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26840.37403807830272556No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26490.3691605325722504No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25250.351880084841424No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23640.32944337448123817No Hit
GTATAATAGTTATTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT23560.328328506885701No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22670.3159256048853498No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21440.2987845156039656No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21310.2969728557612177No Hit
GGTCTATAGGGCGTCTAGTCTCCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA21170.2950218374690276No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19080.26589592153561864No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGAAGGCTGCTGATGGTGAGAGTGAATTCTGTC18570.2587886406140691No Hit
CTATAGACCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC18530.2582312068163005No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17810.24819739845646582No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17370.24206562668101128No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16650.2320318183211766No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16080.22408838670297418No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16060.22380966980408987No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16020.2232522360063213No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16010.22311287755687917No Hit
AGTCTCCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC15990.22283416065799486No Hit
GTAGTAAACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA15730.219210840972499No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14640.20402076998330487No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14550.20276654393832552No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14460.2015123178933462No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14040.19565926301677597No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13310.18548609620749915No Hit
GTGGAGACTAGACGCCCTATAGACCAGGAGCTTAGGGGCTTTCCCTGGTT13280.1850680208591727No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12810.17851817373539175No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12430.17322255265659012No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12240.17057474211718932No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11920.16611527173504056No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT11780.1641642534428505No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11660.16249195204954472No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11460.15970478306070174No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11240.1566388971729745No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11100.15468787888078442No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10990.15315493593692078No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10930.1523187852402679No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10730.14953161625142494No Hit
GAATTCACTCTCACCATCAGCAGCCTTCAGCCTGATGATTTTGCAACTTA10720.1493922578019828No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10510.14646573036369767No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG10430.14535086276816048No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10320.14381791982429687No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10310.1436785613748547No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10210.14228497688043323No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9800.13657128045330516No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9500.13239052697004072No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9140.12737362279012338No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9010.12556196294737548No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT8890.12388966155406969No Hit
CCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAAT8860.12347158620574324No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTGTGGAGACTAGACGCCCTATAGA8660.12068441721690026No Hit
CCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCG8420.11733981443028874No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8400.11706109753140442No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8230.1146920038908879No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8190.11413457009311931No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8130.11329841939646643No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8110.11301970249758211No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTCTCCTTCCACC7990.11134740110427636No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7860.10953574126152843No Hit
CCCTATAGACCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAATAATTAATATTCTGA7830.10911766591320199No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7810.10883894901431769No Hit
ACTCTGGAGGGACGGGTTGTAGTTGGCTCTCCCAGTATGAAAGATTTCCC7810.10883894901431769No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7770.10828151521654909No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7630.10633049692435903No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7570.10549434622770612No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7530.10493691242993754No Hit
CAGTATAATAGTTATTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT7520.10479755398049538No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA7400.10312525258718963No Hit
GTGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTTG7390.10298589413774747No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7390.10298589413774747No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7330.10214974344109456No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7280.10145295119388384No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7250.10103487584555738No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG7230.1007561589466731No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)