FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962097
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAG252.6110306E-455.010161
ACTAGAC200.007471859551.5505833
GTACGTA405.104122E-542.976691
GTATCAA26750.041.6432081
TACTGAC350.001373179439.2786752
GGTATCA10650.039.062381
GTGGTAT3700.039.0274771
TATCAAC29250.037.9526022
TGGTATC4050.035.641762
CTAGACT400.00264439534.370634
TAGGTCC400.00264439534.370634
TTACCTT400.00264439534.370634
ATAAGAG2100.034.3670543
TAAGAGA2150.033.5713124
GTATAAG2050.033.5427781
AACGCAG34700.032.2989586
TATAAGA2150.031.9710162
AGAGTAC34500.031.49296211
CAGAGTA34500.031.29363810
GCAGAGT35650.031.1521859
Produced by FastQC (version 0.11.5)