FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931729
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18700.20070213549218707No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17810.1911500017709012No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT11220.12042128129531227No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10750.11537689607171184No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10260.11011785615774544No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA10190.10936656474146453No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9960.10689803580225579No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9950.10679070845707281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.041.3040541
GTATCAA38350.038.7432141
CTTACGA459.942258E-538.38025735
TATCAAC41900.035.6168172
AACGCAG46850.031.8536746
CAACGCA48200.030.9598455
TCAACGC48750.030.607274
ATCAACG48650.030.6029593
TATTAGC450.004750991230.494912
AATGGTA1702.2842323E-730.1902770
AGAGTAC48050.029.988811
ACGCAGA50650.029.533187
GCAGAGT50100.029.1725949
CAGAGTA49500.029.1103410
CGCAGAG51150.028.640828
TATAAGA2300.025.357182
AGTACGG45650.025.25234213
GAGTACG47000.024.67300412
GTGGTAT6350.023.7767261
GTGTATA2053.6379788E-1223.4340481
Produced by FastQC (version 0.11.5)