FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189511
Sequences flagged as poor quality0
Sequence length20-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1700.046.4278681
GGTATCA16600.039.484361
GTATCAA40150.038.803431
TTATACA2300.037.2987022
TATACAC2400.035.7460943
TATCAAC43950.035.4469222
TATAAGA3100.033.207882
ATCAACG51150.030.6598743
GCTTAGG450.004745130.5033321
AACGCAG51700.030.3337086
CAACGCA52650.029.916735
AGAGTAC52100.029.2384111
TAAGAGA3650.029.1453084
CAGAGTA52350.029.09878210
TTAGGAC605.5944145E-428.5968743
TCTTATA605.595791E-428.5956732
TCAACGC55300.028.4210534
TACACAT2900.028.3996565
ATAAGAG3650.028.2051353
GTATAAG3900.028.1569231
Produced by FastQC (version 0.11.5)