FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702361
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA350.001349177339.4183541
GTATCAA14100.038.404941
TCTATAT501.8666408E-434.4812363
TATCAAC16350.033.1104242
CTTATAC958.807547E-932.6757431
TATTAGA751.6751092E-632.182492
GGTATCA6350.032.0468141
CTATATG652.3288885E-531.8288354
TATACAC1052.3406756E-829.5553473
AACGCAG18450.029.5286466
CAACGCA18650.028.8422155
TTAACGG605.356291E-428.8082135
GTATAGC854.433852E-628.4044061
ATCAACG19100.028.3432183
TGGTATC3450.027.9847742
CTAATGT756.183013E-527.5849914
ATAACAC500.00775345827.584993
CAGAGTA19000.027.57909810
AGAGTAC18800.027.5057511
TCAACGC19500.027.4081634
Produced by FastQC (version 0.11.5)