FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702361
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11280.16060117233160726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3550.037.8346061
GTATCAA16850.035.972091
GGTATCA8050.034.2252851
TGGTATC4200.031.972422
TATCAAC18750.031.9527442
ACCGAAT800.00390784731.73231970
GGTATAC450.00466640630.6060753
CAACGCA21250.028.0375525
AACGCAG21200.027.778786
ATCAACG21550.027.6413363
CAGAGTA20850.027.58438910
AGAGTAC21150.027.19312111
TACCGAA600.00856737427.0256269
TCAACGC22200.026.9890374
TACGGAC658.750603E-426.4879134
ACGCAGA23000.025.604797
GCAGAGT23150.025.587659
CGCAGAG24200.024.6197518
GTTCTAA750.002012794422.9594611
GTACAAC1154.6622998E-520.9629861
Produced by FastQC (version 0.11.5)