FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481400
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30010.2025786418253004No Hit
CTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT28040.18928041042257324No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20780.14027271499932498No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20310.13710004050222763No Hit
CTCTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18810.1269744835965978No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17860.12056163088969894No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17750.11981909004995274No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17680.11934656406102336No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16440.11097610368570271No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16330.11023356284595653No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAGAGCCTCCTG15380.10382071013905765No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15380.10382071013905765No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15180.10247063588497368No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15070.10172809504522748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37250.039.132091
ATCGCGT801.6607373E-938.7645837
ACGAGAC6900.038.0980222
CATCGCG1450.037.9818436
TATACAC7650.036.5868643
CGAGACT7100.036.551423
CCCACGA7050.036.3127919
CGAGCCC7000.036.07966615
GGTATCA15300.035.9224431
TCGTATG1201.8189894E-1234.60007540
TATCAAC42600.034.2118382
TCAACGC43200.033.8958364
TCTCCGA7700.033.683911
ATCAACG43800.033.353583
CACGAGA7950.033.0650921
TCCGAGC8000.032.8484513
CAACGCA45400.032.554045
ATCTCCG8000.032.42075310
AACGCAG45500.032.407476
TTATACA8300.031.6643642
Produced by FastQC (version 0.11.5)