FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481400
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58240.3931416227892534No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28840.19468070743890914No Hit
CTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT25720.17361954907519914No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19880.13419738085594707No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18900.1275820170109356No Hit
CTCTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18520.12501687592817606No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16810.11347374105575805No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16790.11333873363034969No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16760.11313622249223708No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16750.11306871877953288No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16170.10915350344268934No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15310.10334818415012827No Hit
GGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAAT15030.1014580801944107No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15020.1013905764817065No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14830.10010800594032672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16500.039.4841541
GTATCAA38500.037.12161
GTGGTAT5900.035.2655941
TATCAAC42650.033.34172
TGGTATC6400.032.502792
TCAACGC44600.032.110074
ATCAACG44600.031.9571633
AACGCAG46400.030.793016
CAACGCA46600.030.731955
GCGTATG450.004887926430.3194181
CGCAGAG49250.028.9418418
AGAGTAC50600.028.23706611
TCGATAG500.00701155228.16130653
CAGAGTA51950.027.70020510
ACGCAGA52100.027.4895527
TGTTAGA9550.026.18686370
AGTACGG37500.025.27871313
GAGTACG38700.024.40758912
CGAATCG1302.0690277E-723.60698916
AACGGAC750.001812540123.3789650
Produced by FastQC (version 0.11.5)