FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131476
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC150.00239205868.732791
GTTATAG150.00239331668.723683
TATTAGC252.6185592E-454.9789432
ACTAATC200.007186988452.06046741
TAGACTC404.4199238E-544.0383761
GGTATCA13750.042.989241
CTTATAC1200.042.9579931
TTATACA1250.041.2342072
TATACAC1250.041.2342073
GTATCAA34650.040.8627851
ACGTTTG350.001373164839.27935823
GTAAGGT350.001374657439.2706763
TAATCTC1009.094947E-1237.98268535
ATGTGTA1009.094947E-1237.7946811
TATCAAC39100.036.5588872
CTAATCT1102.7284841E-1134.5128534
AGCTAAT1400.034.4776532
TGTGTAT1102.910383E-1134.35880312
GTGGTAT5000.033.6790661
TGGTATC5050.033.3411942
Produced by FastQC (version 0.11.5)