FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131476
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19030.16818739416479006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAC306.453375E-445.79281615
GTATCAA38000.041.8611
TAAGAGA1250.041.209894
GGTATCA14350.039.9831241
TATAAGA1300.039.6301462
ATAAGAG1300.039.6248933
GTGGTAT5900.039.5976221
ATACCAT350.001377751539.2527166
TATCAAC41200.038.6810762
GTATAAG1350.038.1741751
TGGTATC6450.035.6773762
TTTGTCA400.00264925634.35827619
GTTTAGA400.002654429334.3446113
TCTATAC400.002655580234.341573
CAGAGTA46300.033.23340610
AGAGTAC46700.032.9487511
AACGCAG48500.032.575716
CAACGCA48750.032.4776735
GCAGAGT48450.032.467559
TCAACGC50900.031.2380144
Produced by FastQC (version 0.11.5)