FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983273
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA70220.3540611907689965No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61240.3087825024593185No Hit
GTGTATTACTGTGCGAGACATGTTATTCGCCTTGAATACGCTTGGGGGAG59710.30106798206802593No Hit
GTATTACTGTCAGCAGTATGGTGGCTCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCACACGAACTGTGG55040.2775210472789172No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCA51600.2601759818239849No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT48990.247015917627074No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA47480.23940224063958923No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT46900.23647778192916455No Hit
GTCTCTGGTGGCTCCATCAGCACTAGTAATTACTACTGGGGCTGGATCCG45450.22916663515310298No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC43560.21963693349327096No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT40090.20214060293262703No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT39690.2001237348564721No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC38650.1948798778584693No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38390.1935689136089686No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37490.18903096043762002No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTGTTAGCGG35300.17798860772067182No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34920.17607258304832468No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34420.17355149795313102No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC33850.17067746094461023No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32980.1662907728789733No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTTGACACGTCCAAGAACCACTTCTCCCTGAAG32210.16240830183237506No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31720.1599376384390853No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG31120.1569123363248529No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31030.15645854100771803No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29640.1494499244430797No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA29410.14829022529929062No Hit
GTGTATTACTGTCAGCAGTATGGTGGCTCACTCACTTTCGGCGGAGGGAC29330.14788685168405963No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29290.14768516487644415No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28920.14581956190600084No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCACTAGTAATTACTACTGGG28250.14244130787844134No Hit
GTGCAGCCACAGTTCGTGTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG28170.14203793426321035No Hit
GTAGTAGGTGGTCCCACTATGATAGACACTTCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAGCC26630.13427299217001393No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26160.13190317218053188No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT26150.13185275047862802No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26070.13144937686339703No Hit
GTATGGTGGCTCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCATCTGTCTT26040.1312981117576854No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC26040.1312981117576854No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25870.13044094282531957No Hit
CTCCTGCGGGGCCAGTCAGAGTGTTAGCGGCAACTACTTAGCCTGGTACC25660.12938208708533822No Hit
GTATTACTGTGCGAGACATGTTATTCGCCTTGAATACGCTTGGGGGAGTTATCGTTCCTTCTTGTGGTTTTTTGA25640.1292812436815305No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25590.12902913517201112No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA25540.12877702666249177No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC25390.12802070113393366No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25160.12686100199014458No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT25030.12620551986539422No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT24740.12474329051018192No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGA24220.12212136201118051No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC24010.12106250627119917No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT23710.119549855214083No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT23220.1170791918207932No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT23170.11682708331127384No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22370.112793347158964No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT22360.11274292545706012No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21670.10926382802569287No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA21660.109213406323789No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC21420.10800328547809605No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21300.10739822505524958No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTGGCTCA21200.10689400803621085No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21180.1067931646324031No Hit
CTGCTACTCTGGCTCCCAGGTACCACCGGAGAAATTGTGTTGACGCAGTC21080.10628894761336438No Hit
GTCTGGGACGCCAGTGGCCCTGCTGGATGCATCATAGATGAGGAGCCTGGGCGCCAGGCCAGGTTTCTGCTGGTA20530.10351575400865136No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA20420.10296111528770874No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT20370.10270900677818938No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA20250.10210394635534292No Hit
CTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCTCCTCATCTATGATGCATCCAGCAGGGCCA20110.1013980425286887No Hit
GCCACAGTTCGTGTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGA20080.10124677742297708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAT21500.043.3252570
GTGGTAT5500.043.0136261
GTATCAA31100.037.2628441
TGGTATC6350.037.2493252
GGTATCA14400.033.571931
TATCAAC35350.032.6801072
GTTATTA1053.334876E-431.23718670
TGTTAGA10800.030.97687770
TTTTTTG24700.030.80720170
ATCAACG38200.030.2388843
TCAACGC38600.029.9255284
AACGCAG39000.029.8845256
CAACGCA39300.029.65645
CAACGGA23700.028.47670669
AGAGTAC40450.028.30479411
CGCAGAG41500.027.9190528
TTTAACG1005.0595554E-727.5757134
GCAGAGT42050.027.553889
GTATTAG1405.0386006E-1026.9391771
TAACGGC1057.6656215E-726.29458236
Produced by FastQC (version 0.11.5)