FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791399
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC44200.24673453541059248No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT42180.23545843220856996No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT39510.22055387995639164No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC39180.21871174428477408No Hit
GTCTCTGATGGCTCCATTAGCAGTAGTAATTACTACTGGGGCTGGATCCG33920.18934921812505198No Hit
GTTTATTACTGCGCGAGAGGTCGGTTTGTGATCGACAGTGGCTGGTATCC33000.18421356716175458No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG32200.17974778371540903No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30870.17232341873585952No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA29550.16495487604938933No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28720.16032162572380582No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC27830.15535344163974638No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25780.14390987155848584No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC25520.14245849193842355No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA25310.14128622378375782No Hit
GTATAATAACTGGCTACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT25050.13983484416369554No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT24980.1394440881121403No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT24760.13821599766439527No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24140.13475501549347746No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC22660.12649331611773815No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA22500.12560015942846903No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT22040.12303233394682032No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21680.12102273139596484No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG20610.11504974603647763No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20430.11404494476104987No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA19910.11114218552092527No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT19810.11058396259013208No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19600.10941169443546635No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19520.1089651160908318No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA19500.10885347150467316No Hit
CACCTGCACTGTCTCTGATGGCTCCATTAGCAGTAGTAATTACTACTGGG19390.10823942628080065No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC19290.10768120335000746No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG18370.10254555238671005No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA18350.10243390780055141No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18330.10232226321439278No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT18020.10059177212893386No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCTTTAGACACGTCC18020.10059177212893386No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT18010.10053594983585455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA350.001375062239.2695724
GTATCAA26750.039.1679731
GGTATCA10850.038.309871
TCGTATG2750.037.79063840
CGTATGC3050.034.09752341
CTCGTAT3100.033.49848639
TATCAAC31450.033.2016372
TAAAGAT14050.031.37747270
AACGCAG33950.031.3637856
ATCAACG34100.031.1250933
CAACGCA34250.031.0890665
TCAACGC34750.030.5428964
CTTATAC3650.029.1758751
TGTTAGA5400.028.3963670
AGAGTAC36800.028.37080811
CGCAGAG37800.028.2570388
TATACAC4550.027.9316673
GCAGAGT39600.027.1453299
CAGAGTA39300.026.56605110
ACGCAGA41050.025.936947
Produced by FastQC (version 0.11.5)