FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791399
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT45920.2563359698202355No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT45680.25499623478633177No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC39580.22094463600794687No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC39410.21999565702559842No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32730.18270636524861297No Hit
GTCTCTGATGGCTCCATTAGCAGTAGTAATTACTACTGGGGCTGGATCCG31700.17695666906144303No Hit
GTTTATTACTGCGCGAGAGGTCGGTTTGTGATCGACAGTGGCTGGTATCC31000.17304910854589067No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29610.16528980980786526No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28100.15686064355288798No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27960.15607913144977753No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA27910.15580001998438092No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC26830.14977121233181442No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25400.14178862442147172No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA25040.1397790218706162No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC24750.13816017537131595No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT24410.13626221740661906No Hit
GTATAATAACTGGCTACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT24400.13620639511353977No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC24230.1352574161311913No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT23410.1306799880986871No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT23110.12900531930630754No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT22940.1280563403239591No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT22890.1277772288585625No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21750.12141348744752008No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21640.12079944222364757No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT20930.11683605941501586No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA20630.11516139062263628No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20630.11516139062263628No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19690.10991409507318023No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT19530.10902093838391112No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA19530.10902093838391112No Hit
CACCTGCACTGTCTCTGATGGCTCCATTAGCAGTAGTAATTACTACTGGG18910.1055599562129933No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC18800.10494591098912079No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18680.10427604347216897No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA18660.10416439888601033No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG18570.10366199824829644No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA18490.1032154199036619No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG18030.1006475944220132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.036.9245871
GTATCAA30750.036.6266861
TATCAAC34650.032.494222
ATCAACG35950.031.1282163
CGTATAT802.3414977E-630.83486262
AACGCAG36900.030.5145686
TCAACGC36750.030.3563394
TGTTAGA5550.030.2854370
CAACGCA36750.030.2646245
AGAGTAC39450.028.1101911
CGCAGAG40300.027.7728738
TAAAGAT12450.027.5207370
ACTCGTA657.8020134E-427.01621659
GTGGTAT8450.026.8198381
GCAGAGT42100.026.5854369
CAGAGTA43000.025.94915210
TTAGACT809.899165E-525.7445134
ACGCAGA44250.025.448897
TCGTATA700.001184847725.14969661
AGTACGG38450.024.37613
Produced by FastQC (version 0.11.5)