FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973293
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20670.21237181403749952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATAAT100.009669699130.701170
GTTCATA200.00742538951.632042
GTATAAG900.045.9116631
TATAAGA950.043.479612
ATAAGAG1000.041.2992673
GTATCAA33850.040.0794531
TAAGAGA1050.039.332634
GGTATCA13850.038.5359571
TATCAAC36250.037.4124572
GTGGTAT5350.036.0427971
CCTTTAG501.8823516E-434.4337431
TGGTATC5800.033.2344172
AGAGTAC39950.032.3949111
CAGAGTA39950.032.3949110
AACGCAG41950.032.163246
GCAGAGT41900.031.9551819
CAACGCA44150.030.7944245
ACGCAGA43850.030.769627
CTTTAGG450.004674232630.5967662
ATCAACG44700.030.1814163
Produced by FastQC (version 0.11.5)