FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979174
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTA150.002570241267.49782624
TTACTCA252.1447595E-457.24436655
TATCGTA200.007570415751.3835
TATACAC1900.049.679363
TTATACA1750.046.232222
CTTATAC1850.043.7354131
TAATCTC1700.042.31294335
CTAATCT1700.042.2296634
CTAAGAG400.001012017741.79240869
AGGGGGG2750.039.92680470
ATACACA2200.039.8402634
TACACAT2350.038.7317735
GTATCAA41600.037.5216031
AGCTAAT2200.037.15900432
TGACTAA400.001847003136.97840566
GCTAATC1950.036.76228733
GGTATCA17650.036.4823151
CAGCTAA2250.036.26026531
TATCAAC44450.035.1140372
ATGTGTA808.707502E-833.70410511
Produced by FastQC (version 0.11.5)