FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979174
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27070.2764575039778425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAA705.5479177E-1043.3505742
TTACGGG405.7285135E-542.1442383
GTGTATA754.9087248E-835.9741061
GTATCAA43400.035.2799031
GGTATCA20150.034.311531
TATCAAC45800.033.422642
GGATTAT450.00424447431.20580547
CGAATAA450.004311155531.10656745
AGAGTAC49050.029.97229811
CAGAGTA49250.029.91904410
AACGCAG51600.029.7326936
GCAGAGT51600.029.3406129
AGGAGTA1203.428795E-928.0975952
TATGCAC606.2025903E-428.096164
ATCAACG54300.028.0651153
AGTCAAA500.007168317728.03305246
ACGCAGA55050.027.9305887
CAACGCA55150.027.8799425
CGCAGAG55050.027.624338
TCAACGC56950.027.0551954
Produced by FastQC (version 0.11.5)