FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740990
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGAAGGCTGCTGATGGTGAGAGTGAATTCTGTC77090.4427940424700888No Hit
GTATAATAGTTATTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT73570.42257566097450305No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT71260.4093073481180248No Hit
GGTCTATAGGGCGTCTAGTCTCCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA66920.3843790027513082No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54610.313672106100552No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46900.26938695799516366No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46790.2687551335734266No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46200.2653662571295642No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44430.255199627797977No Hit
AGTCTCCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC42120.2419313149414988No Hit
CTATAGACCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC42070.24164412202252744No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC41070.23590026364309963No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40340.2317072470261173No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA40080.2302138438474661No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37950.2179794254992849No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT35980.20666402449181212No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34440.19781848258749332No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG33000.18954732652111728No Hit
GAATTCACTCTCACCATCAGCAGCCTTCAGCCTGATGATTTTGCAACTTA32410.1861584500772549No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32030.18397578389307234No Hit
GTAGTAAACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA31230.1793806971895301No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30500.1751876805725478No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30280.1739240317290737No Hit
GTGGAGACTAGACGCCCTATAGACCAGGAGCTTAGGGGCTTTCCCTGGTT29270.1681227347658516No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28370.1629532622243666No Hit
CCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCG28090.16134498187812682No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28000.1608280346239783No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27840.15990901728326987No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA27320.15692221092596742No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT27290.1567498951745846No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAATTATTGGTTGGCCTGGTATCAGCAGAAA26900.15450979040660773No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26860.15428003607143062No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26860.15428003607143062No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26610.15284407147657367No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26530.15238456280621945No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25800.14819154618923716No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25410.14595144142126032No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25390.14583656425367178No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25190.1446877925777862No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24980.14348158231810634No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24880.1429071964801636No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24740.14210305630704367No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24730.14204561772324942No Hit
CCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAAT24550.14101172321495242No Hit
GGCCAAAAGTGTACGAATAACTATTATACTGTTGGCAGTAATAAGTTGCA24070.13825467119282708No Hit
GTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACT23630.13572737350587882No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22100.1269392701853543No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21540.12372270949287474No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCACTGTGGAGACTAGACGCCCTATAGA21190.12171235906007502No Hit
CAGTATAATAGTTATTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20460.11751934244309271No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20150.1157387463454701No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19980.11476229042096737No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19950.11458997466958455No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19940.11453253608579028No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19820.11384327308025895No Hit
GTGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTTG19710.11321144865852188No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTCTCCTTCCACC19540.11223499273401916No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGGTCTATAGGGCGTCTAGTCTCCACAGTGGGGTCCCAT19470.1118329226474592No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18970.1089609934577453No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18870.10838660761980252No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGAAGGCTGC18340.1053423626787058No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18210.10459566108938018No Hit
CCCTATAGACCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAATAATTAATATTCTGA18160.10430846817040879No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18150.10425102958661452No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17940.10304481932693467No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17890.10275762640796328No Hit
GGCTGAAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCA17790.10218324057002051No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17710.10172373189966628No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT17460.10028776730480933No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17430.1001154515534265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31550.040.806311
GTGGTAT5050.036.03131
TATCAAC37550.034.1896552
ATCAACG39650.032.3769953
TCAACGC39450.032.2801134
CAACGCA40000.031.9220735
AACGCAG40500.031.4450266
CACTGAA13800.031.08697170
AGAGTAC43100.029.31001911
CGCAGAG43450.029.231938
CAGAGTA43400.028.94922410
GCAGAGT44450.028.4970659
ACGCAGA46900.027.15487
TGTTAGA14750.026.88136770
AGTAATA25700.025.25073417
CCTATAC1501.1077645E-925.1715243
GTCCACT9550.025.0226167
ATCAGGC25950.025.01746636
AGTACGG39700.024.64335813
TAATAAG26350.024.63563719
Produced by FastQC (version 0.11.5)