FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805032
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA950.057.9354252
CTTATAC1000.055.038661
CCCATTA200.00721439652.00940
CTATAGA200.007443886751.5987431
GCCTAAA200.007443886751.5987431
CGTAACA200.007443886751.5987434
TATACAC1200.051.598743
TCGTTTT400.00921767448.87854870
GACTAGC1150.047.90433526
AGACTAG1200.045.9026125
ATACACA1400.044.2274934
GTATCAA22750.043.547071
GAGACTA1300.042.3584824
TACACAT1550.042.1667145
GGTATCA8450.041.930341
ACTAGCG1450.040.38021527
GTGGTAT2750.040.0281141
AATTCTC350.001317737439.60854739
TATCAAC26000.038.3682942
CTAGCGC1600.036.61278528
Produced by FastQC (version 0.11.5)