FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805032
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16910.21005376183803873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAAG200.00727753551.89451636
TTAGAGC200.00744984251.5883063
TCTATAC200.00744984251.5883063
TATAGCA306.406422E-445.8591232
GGTATCA9850.044.357021
GTGGTAT2800.044.232291
GTATCAA25750.042.4858971
TGGTATC3000.042.419692
TATCAAC28500.038.3768462
CTACTTG400.002524714534.69606442
GGTATGT400.002631201934.40502516
GTGTATA601.3682062E-534.402891
TGTATAA601.37053685E-534.394342
TATACCG501.8947686E-434.394345
CTAGATC400.002636015934.3922044
CCTAGAC501.8954604E-434.3922043
AACGCAG35000.031.7430366
CAACGCA34900.031.733465
CAGAGTA34350.031.2441610
AGAGTAC34500.031.10831311
Produced by FastQC (version 0.11.5)