FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194045
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.044.1945651
CTTATAC1450.042.744591
GTATCAA40100.041.0449941
TATACAC1650.037.555563
TATCAAC44750.036.770742
GTGGTAT4650.036.2843861
TTATACA1750.035.4080472
TACACAT2050.033.5862775
AACGCAG50850.032.2933546
CAACGCA52150.031.686385
ATCAACG53900.030.5298613
GCAGAGT54100.030.2871919
CAGAGTA52700.030.24137510
TCAACGC54650.030.2368684
AGAGTAC52600.030.10254711
TGGTATC5900.029.1732962
ACGCAGA56650.029.1681467
CGCAGAG56200.029.1554648
TAATTAG605.234942E-428.92327741
ATACACA2450.026.6976624
Produced by FastQC (version 0.11.5)