FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194045
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18360.15376304913131417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44300.041.949731
GGTATCA17650.038.021481
TATCAAC49600.037.527152
GTGGTAT5950.035.8602941
TATAGCC709.94738E-734.4056662
TTGTACC709.953892E-734.4027824
AACGCAG55400.033.788876
CAACGCA56650.033.0419245
AGAGTAC56200.032.33095611
CAGAGTA56350.032.24489210
ATCAACG58100.032.034263
GCAGAGT57800.032.0313579
TCAACGC59150.031.4656094
CGCAGAG59850.031.0492088
ACGCAGA61050.030.664387
GTACTAA802.8158156E-630.1125261
TGGTATC7650.027.884332
TCCGTAA550.009033862526.73539468
GAGTACG68650.024.41131412
AGTACGG67350.024.37156913
Produced by FastQC (version 0.11.5)