FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136845
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCATA350.005523146655.6410570
TAAGGTG350.005523146655.6410570
AGTAACA200.007407675551.663827
TAATATC306.2537665E-446.0857335
TTATACA1050.042.6018642
GGTATCA14750.042.0019721
TATACAC1150.041.8913043
GTGGTAT4950.041.719131
GTATCAA36800.039.655851
GCTAATA350.001343964439.45149633
CAGCTAA1350.038.31672731
TACACAT1500.036.704765
TTCTTAT4650.036.2963170
TATCAAC41250.035.785572
AGCTAAT1450.035.69307732
TATACTG501.8899664E-434.4107135
TATAACG400.002629525334.410713
GAGGATA400.00263236334.4031413
CTTATAC1400.031.9598351
TAAACTA3400.031.3744755
Produced by FastQC (version 0.11.5)