FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127583
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21390.18969778721389025No Hit
GCGTAACACCTGGCCTCCAACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19680.17453260646888077No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17740.1573276645710338No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA17700.1569729235009751No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17400.15431236547553484No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT14440.128061526291191No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14240.12628782094089747No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13780.12220829863522242No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13600.12061196381995827No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13050.11573427410665113No Hit
GTGTTACGCTGCTTACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC12840.11387188348884296No Hit
GTAATATACAGCCGTGTCTTCAGATCTCAGGCCGCTCAGCTCCATGTAGA12710.11271897501115218No Hit
GTACCAACAGAGGCCTGGCCAGGCTCCCAGGCTCCTCATCTTTGATACAACCAACAGGGCCCCTGGCATCCCAGC12520.11103395492837334No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTTA12310.10917156431056517No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTT11750.10420518932974336No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11340.10056909336164166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.037.036171
TATACAC2200.035.8513873
TATCAAC26500.030.1516322
CTTATAC2600.029.0219881
TGTTAGA4650.028.74680770
GTGGTAT4550.028.6450791
TTATACA2650.028.4693532
CAACGCA28300.027.991515
AACGCAG28700.027.7208716
GTCGTAT756.0417922E-527.6808539
GTATCAA30500.027.3265151
TCAACGC31350.025.8151864
TCGTATG1901.8189894E-1225.5240740
CACTAGT4500.025.46145870
CGCAGAG31150.025.3181538
AGAGTAC30900.025.30105611
ATCAACG32000.025.183653
CGTATGC2250.024.65373841
ACGCAGA32350.024.5909867
TGGTATC5600.024.4947362
Produced by FastQC (version 0.11.5)