FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497172
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGTTATCCGTGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT104040.2974975208539929No Hit
CCATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGGC102480.2930367737131602No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT84830.24256742304925236No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74980.21440180808950776No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC71030.20310696757265584No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC70920.20279242771016123No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC65160.1863219767286253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC62930.17994539587987093No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC59770.1709095234663894No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT54480.15578301553369409No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCAGTTTCAGGGAAA53410.15272340050761016No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT51020.1458893071315909No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT50330.14391628435776108No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC49630.1419146670509772No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49140.14051353493622848No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA48210.13785424337150132No Hit
GTGTATTACTGTGCGAGATCACTATCCTCCGGATATGGCAGTCCCTGGCT47630.1361957604601661No Hit
GTCTCTGGTGGCTCCATCAGTAGTCACTGCTGGAACTGGATCCGGCAGCC45250.1293902616171009No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT45210.12927588348528468No Hit
GAGTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGAGGCGT43880.12547281060239532No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC43850.12538702700353313No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT43840.12535843247057907No Hit
CCTTCCACCCTGTCTGCTTTTGTAGGAGACAGAGTCACCATCACTTGCCG43550.12452919101491149No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTTGGCCGAACGTC43160.12341400422970332No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG42370.12115503612633294No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG42040.1202114165388491No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG41040.11735196324344356No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39710.11354889036055418No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT39250.11223354184466763No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT38870.11114694959241352No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCCGGTGCCAAATGTGACAT38500.11008895187311347No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCGGGGAGCCAGAGCAGCAGGAGCCCCAGGAG38160.10911673775267558No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT37870.108287496297008No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36190.10348361476072666No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC35630.10188232091529956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)