FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083553
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14380.13271155171920523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTA350.006569907553.2527470
GTATCAA52600.035.7257231
GGTATCA24100.034.5920941
TCGTATG1900.032.85069340
TATCAAC58300.032.5213362
AACGCAG63700.029.3890176
TCAACGC65150.028.9446724
ATCAACG66050.028.550273
CAACGCA65950.028.489965
GTGGTAT8450.028.3038431
AGAGTAC68650.026.9688611
ACGCAGA69650.026.9274447
TGGTATC9150.026.8816152
CAGAGTA70050.026.62492410
CGCAGAG71400.026.3153088
ACTTAGG659.168869E-426.2785022
GCAGAGT71150.026.2613039
TACGGTC1209.369069E-825.621544
CTCGTAT2500.024.93265739
ACGCGTA2500.024.6376127
Produced by FastQC (version 0.11.5)