FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096689
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23200.036.4616741
GGTATCA11050.031.7414951
TATCAAC28350.030.1978722
GTGGTAT5550.029.7397821
GTGTTAG1750.027.5092961
CTCGTAT1404.5110937E-1027.19670739
TCAACGC31800.027.1379284
CAACGCA31600.026.9832765
ATCAACG31950.026.7952963
AACGCAG31950.026.7952966
ATTAGAG1057.916351E-726.1957453
TATTAGA1057.916351E-726.1957452
AGAGTAC32300.025.64402211
GTGTATA1759.094947E-1225.5443461
CAGAGTA32950.025.13814710
CGCAGAG34050.024.9328148
ACGCAGA34250.024.8887087
TGGTATC6750.024.4493622
GCAGAGT34850.024.3593569
TCGTATG2200.023.61563140
Produced by FastQC (version 0.11.5)