FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096689
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18540.16905430801257237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24800.035.5923461
GTGGTAT6800.033.3357471
GGTATCA12550.029.830321
TATCAAC29600.029.6991622
TGGTATC7700.028.9880222
TCAACGC32250.027.2562794
AACGCAG32500.027.0503166
CAACGCA32450.026.984945
ATCAACG33000.026.532773
AGAGTAC32050.026.14671511
CGCAGAG33450.025.9764488
CAGAGTA32750.025.7975910
ACGCAGA34550.025.348227
GCAGAGT35150.024.8178259
TCTAATG1001.6210035E-524.037762
TTAACGG1253.486186E-622.09221835
CTAGTTA953.1470213E-421.7050324
GAGTACG35850.021.26763312
TTTAACG1304.923253E-621.22986834
AGTACGG35950.021.20750813
Produced by FastQC (version 0.11.5)