FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691955
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT72040.4257796454397428No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGAAGGCTGCTGATGGTGAGAGTGAATTCTGTC69210.40905343227213486No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT67810.4007789805284419No Hit
GGTCTATAGGGCGTCTAGTCTCCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA57920.34232588928192537No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43180.25520773306618677No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41490.24521928774701454No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39120.2312118230094772No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38550.22784293908525935No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC38360.22671997777718675No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38160.2255379132423735No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37560.22199171963793365No Hit
AGTCTCCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC36720.21702704859171787No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA31610.1868252997272386No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30850.18233345449494817No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAATTATTGGTTGGCCTGGTATCAGCAGAAA29600.17494555115236515No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG29300.17317245435014525No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28890.17074922205377802No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28790.1701581897863714No Hit
CCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCG28790.1701581897863714No Hit
GAATTCACTCTCACCATCAGCAGCCTTCAGCCTGATGATTTTGCAACTTA28430.1680304736237075No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA28190.16661199618193157No Hit
CTATAGACCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC28050.16578455100756226No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27970.16531172519363696No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27910.16495710583319295No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT26320.1555596927814274No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25690.15183618949676558No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24950.14746255071795644No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24210.1430889119391473No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23200.13711948603834026No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22680.13404611824782572No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22660.1339279117943444No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22590.13351418920715977No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22560.1333368795269378No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22250.1315046794979772No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22030.1302044085096826No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21950.12973158269575727No Hit
GGCCAAAAGTGTACGAATAACTATTATACTGTTGGCAGTAATAAGTTGCA21930.12961337624227595No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21670.12807669234701868No Hit
GTAGTAAACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA20830.12311202130080293No Hit
CCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAAT20410.12062968577769503No Hit
GTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACT20250.11968403414984441No Hit
GTGGAGACTAGACGCCCTATAGACCAGGAGCTTAGGGGCTTTCCCTGGTT19730.11661066635932989No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19400.11466025987688798No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTCTCCTTCCACC19390.1146011566501473No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19210.11353729856881536No Hit
CAGTATAATAGTTATTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19180.11335998888859337No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19070.11270985339444607No Hit
GTGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTTG18600.10993200173763486No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18550.10963648560393155No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18250.10786338880171163No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18000.10638580813319502No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17920.10591298231926972No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17620.10413988551704981No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17450.10313513066245851No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17030.10065279513935065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6200.041.5260541
GTATCAA45400.041.2089651
TATCAAC49650.037.8142052
ATCAACG54000.034.764983
AACGCAG53850.034.6130266
CAACGCA53850.034.6109775
TCAACGC54150.034.5419164
AGAGTAC58800.031.40728611
CGCAGAG59150.031.395548
CAGAGTA59250.031.22668310
TAGTACG553.3590884E-431.200084
ACGCAGA60750.030.7946767
GCAGAGT60500.030.6949759
TGTTAGA11300.028.10567570
CACTGAA12100.027.85443570
AGTACGG47400.026.14138613
GAGTACG47900.026.08425712
GCGGTCA7600.025.32815623
GAGTACT24500.024.65807512
TAAACGG7550.024.549725
Produced by FastQC (version 0.11.5)