FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503935
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA150.002350790469.029492
CTCTTAT252.5591315E-455.229071
TAATACG200.00734423151.7721142
TAGTCTG200.00734423151.7721145
TACGTAG200.00734423151.7721145
GCGTTAA306.310209E-445.99682619
GTATCAA13700.044.344511
GGTATCA4850.043.4146041
GTGGTAT1400.041.914921
TATCAAC15100.040.2291032
AGCGTTA350.001347555439.4258518
GTATAAG655.420716E-737.1734121
ATAAGAG655.424936E-737.169723
TATAAGA655.424936E-737.169722
TGGTATC1700.036.545022
CAGAGTA17300.034.6970310
TACTACT501.8552747E-434.5147444
TAAGAGA709.689375E-734.5147444
TAGGGTG400.00259583634.497629
AGAGTAC17450.034.3987711
Produced by FastQC (version 0.11.5)