FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503935
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7830.15537718158095787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGACA150.002359994568.9614943
GTGGTAT1300.050.4099461
GTATCAA13650.045.2299771
GGTATCA4850.041.958151
TATCAAC14700.041.7522052
TAGAATG503.853247E-641.37695
TGGTATC1700.040.5655862
CACAAAG350.001279836239.8414453
GAAAACA400.001305844539.67766669
TTAGGCT350.001350799139.4065744
TATAAGA754.0328814E-836.779472
TAAGAGA754.0328814E-836.779474
GTTTGAA400.002480290834.8193451
TTTAGGC400.002602068734.480753
CTAGAAT501.8660494E-434.480754
CTACCCT400.002602068734.480754
CAACGCA18250.034.008415
CAGAGTA18300.033.72707410
AGAGTAC18350.033.63517411
AACGCAG18950.032.7521636
Produced by FastQC (version 0.11.5)