FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860470
Sequences flagged as poor quality0
Sequence length20-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC1050.045.8602943
GGTATCA9900.040.6536251
GTATCAA21450.037.5264241
AGACTAG1051.6370905E-1136.04775225
CTTATAC1102.910383E-1134.399221
GAGACTA1154.9112714E-1132.9074324
TATCAAC25700.031.1832182
TGGTATC3600.029.6181092
TCGTATG1301.8735591E-1029.33377840
TTAATCG1202.5993359E-928.83528137
GTGGTAT3700.028.8209691
TAGGCAT1202.7266651E-928.70605929
AACGCAG28400.028.3397256
ATACACA1700.028.3254784
CAACGCA28550.028.3113045
TCAACGC29100.027.7762134
CAGAGTA29200.027.09055310
AGAGTAC29400.027.02324911
ATCAACG30100.026.8534153
TAATCGT1306.0535967E-926.63898738
Produced by FastQC (version 0.11.5)