FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860470
Sequences flagged as poor quality0
Sequence length20-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13620.15828558810882426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAT400.0097177748.2298770
GGTATCA10400.038.670241
GTGGTAT4100.038.5654451
TGGTATC4300.036.7610172
GTATCAA22000.035.935981
TATCAAC25750.030.8271162
ATAAGAG1251.3096724E-1030.2399353
TATAAGA1254.327376E-927.490852
TATTAGG500.00788484927.4908492
CAACGCA28800.027.0883145
AACGCAG28650.026.9902256
GTGTATA907.1032373E-626.734981
GTATAAG1306.553819E-926.4411891
AGAGTAC28550.026.1217511
TCAACGC30300.026.084554
CAGAGTA28750.025.82049210
ATCAACG30950.025.5367343
ACGCAGA30750.025.2587557
CGCAGAG30850.024.9540778
ACTTAAT700.001348169924.59685529
Produced by FastQC (version 0.11.5)