FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562092
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8440.15015335567842986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17500.036.546341
TGGTATC4250.034.786432
GTGGTAT4350.033.199311
CGAGTGA1002.8389273E-432.09742470
TATCAAC19900.031.963112
AAACGCG450.00438824630.99186150
CAACGCA21000.030.4498655
AACGCAG20950.030.358446
TCAACGC20950.030.358444
GGTATCA8200.029.7723771
ATCAACG21700.029.467613
ACGCAGA21950.028.9753677
CGCAGAG22300.028.6747638
CAGAGTA22450.028.33003610
AGAGTAC22600.028.14200411
ATATACT500.00786543527.5031035
GCAGAGT23650.027.1833029
ATAGCTA950.00855489927.02941170
AGTACGG22000.025.1590913
GAGTACG22750.024.63190312
Produced by FastQC (version 0.11.5)