FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645247
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10300.15962879331480812No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8740.13545200520110903No Hit
GTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTT7240.11220509355332144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14250.039.8993451
TATCAAC15250.037.7232132
GGTATCA5850.035.3420831
GTGGTAT2450.035.1617621
CAACGCA16500.034.87085
AACGCAG16650.034.556656
TCAACGC16700.034.2415734
ATCAACG16850.034.141193
ACGCAGA17850.032.2335137
TTTAACG652.2911803E-531.90432734
AGAGTAC17700.031.33877411
CAGAGTA18000.030.8164610
CGCAGAG19050.030.2030548
TTAACGG703.792721E-529.63466335
TGGTATC2700.028.0686152
TAACGGC756.054331E-527.66977936
AGTACGG16650.026.0706613
GAGTACG17250.025.36356712
GCAGAGT23650.024.4741449
TGTTAGA1901.901041E-523.5401370
Produced by FastQC (version 0.11.5)