FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664719
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68350.4105798035584384No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTTCGAAGATT48300.2901390565014276No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACATTA47910.2877963187781241No Hit
GTCCCAGACCCACTGCCACTGAATTTGGCTGGGATACCAGTGGCCCTGGG46820.28124866719248115No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45670.2743405944186376No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44060.26466929253525673No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42110.25295560391873945No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40380.24256345965895745No Hit
CTGGTATCCCAGCCAAATTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT39330.23625608886544816No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAAAGTC38530.23145047302277438No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38310.23012892866603912No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37030.22243994331776115No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTTCGAAGATTCTGCAGTTTATTACTGTCATCAATATGATAACTGG36310.21811488905935478No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC36080.21673327450458607No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT34240.20568035806643645No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33990.2041786031156009No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33860.20339769054116638No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAATAACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT33040.19847193430242582No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31680.19030238736988045No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31450.18892077281511174No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29720.17852862855532978No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29500.1772070841985945No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28720.17252160875198758No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC28390.17053929221688466No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27500.16519304459191012No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAATTTGGCTGGGATACCA27100.16279023667057324No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26300.15798462082789946No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGAATAACTTAGCCTGGTA26260.15774434003576582No Hit
GGATACCAGTGGCCCTGGGGGAGGCACCAAAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGG25020.1502956354796215No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24770.14879388052878595No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23860.14332749250774454No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC23650.1420660183490427No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23460.14092468458640767No Hit
ATATGATAACTGGCCTCAGACTTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23360.14032398260607346No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22850.13726040250636892No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22730.13653956012996787No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22120.13287527804992913No Hit
CTCCCAGGCTCCTCATCTTTGGTGCCTCCCCCAGGGCCACTGGTATCCCA21090.1266880476524867No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA20970.12596720527608563No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20900.12554671388985167No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20850.12524636289968458No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20840.12518629270165116No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20780.12482587151345062No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA20770.1247658013154172No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19890.11947962388847606No Hit
GTAATAAACTGCAGAATCTTCGAACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA19810.1189990623042087No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19810.1189990623042087No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC19640.11797786893764052No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19150.11503442923400285No Hit
TTGTAAGGACCACCTGGTTTTTGGAGGTGTCCTTGGTGATGGTGAGCCTG18930.11371288487726756No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18900.1135326742831673No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18790.11287190210479967No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18750.11263162131266598No Hit
GTTATCATATTGATGACAGTAATAAACTGCAGAATCTTCGAACTGCAGGCTGCTGATGGTGAGAGTGAACT18690.11227120012446544No Hit
GTTTATTACTGTCATCAATATGATAACTGGCCTCAGACTTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA18680.11221112992643202No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18450.11082951537166331No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17860.10728537368769145No Hit
CCCCAGGGCCACTGGTATCCCAGCCAAATTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAG17660.10608396972702298No Hit
CTGCTACTCTGGCTCCCAGACATTACTGGAGAAATAGTGATGACGCAGTC17320.10404158299388666No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17310.10398151279585324No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17180.10320060022141875No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA16880.1013984942804161No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA16860.10127835388434926No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA16730.10049744130991477No Hit
GCCCTGGAGTGCCTTGCAATCATTTATTGGAATGATGATAAGCGCTACAG16680.10019709031974765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTTT2300.038.94258570
GGTATCA11700.037.8928071
GTATCAA25100.037.2432981
TCGTATG2200.036.1964840
TATACAC3000.034.3689273
CTTATAC2950.033.785391
CTCGTAT1850.033.6665939
GTGGTAT4100.032.6914141
TTATACA3000.032.0767022
TACACAT3300.031.244485
TAGTACG553.3311744E-431.244484
AATCTCG2250.030.69678536
TATCAAC30350.030.6876412
ATCAACG31450.029.5059173
TGTTAGA9550.028.80644470
TCAACGC32400.028.7468514
TGCTAGC605.546347E-428.6399122
CAACGCA32750.028.4396345
AACGCAG33150.028.2001466
ATACACA3900.027.3188934
Produced by FastQC (version 0.11.5)